Isolation, Structure elucidation and Biological Activity of Di- (2-ethylhexyl) phthalate Produced by Penicillium janthinellum 62
نویسندگان
چکیده
On the course of screening program for new bioactive compounds, the present study was carried out on the crude extract of Penicillium janthinellum 62. The crude extract of P. janthinellum 62 was fractionated on silica gel column and Sephadex-LH20, ten active fractions were produced. Fraction 7 showed highest antitumor activity, which then purified using preparative TLC and produced six sub-fractions. Sub-fraction (A) showed highest antitumor activity. Sub-fraction (A) was subjected to many identification instruments including IR, NMR, MS and UV spectroscopy. The obtained results showed that, the suggested chemical structure of the compound may be di-(2-ethylhexyl) phthalate (DEHP) or one of its isomers. DEHP was evaluated for antioxidant, antitumor, antiviral activities and cytotoxicity against two tumor cell lines. Results showed that DEHP has antioxidant activity and the maximum activity was 77.99 % at maximum concentration 12 μg/ml, has antitumor activity using Ehrlich cells and the maximum antitumor activity was 77.29 % at maximum concentration 12 μg/mL. also, DEHP showed cytotoxicity against human breast aden carcinoma cell line (MCF-7) and human alveolar basal epithelial cell line (A-549). In addition, DEHP showed antiviral activity against H1N1 at different concentrations.
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Isolation, structural identification and biological activity of two metabolites produced by Penicillium olsonii Bainier and Sartory.
From the culture broth of a fungus, two metabolites have been isolated: bis(2-ethylhexyl)phthalate (DEHP) precedently isolated from Streptomyces sp. and 2-(4-hydroxyphenyl)-2-oxoacetaldehyde oxime (PHBA) here reported as a natural compound in the (E)-s-cis configuration. The producing organism was identified as a strain of Penicillium olsonii. Culture growth and chemical identification are disc...
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